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作者：羅文琦 甄文俊王抒 王永忠 王懷斌 作者單位： Department of Cardiothoracic Surgery, Beijing Hospital, Beijing 100730, China
衛生部北京醫院心胸外科,100730
關鍵詞：一氧化氮 雌二醇 心血管藥物 硝酸甘油 藥代動力學 英文關鍵詞： Nitric oxide Estradiol Cardiovascular agents Nitroglycein Pharmacokinetics 分類號： R5 刊名：《中華胸心血管外科雜志》 英文刊名： CHINESE JOURNAL OF THORACIC AND CARDIOVASCULAR SURGERY 期刊類別： R6 出版年份/卷/刊期： 2008/24/04 欄目：臨床研究 頁碼： 255-257 摘要：目的 觀察17β雌二醇(E2)、辛伐他汀和異搏定一硝酸甘油復合溶液(V-C)在短時間內對體外培養的人臍帶靜脈內皮細胞(HUVECs)合成一氧化氮(NO)功能的影響.方法 HUVECs分別用17β-E2(10-5mol/L)、辛伐他汀(10-9mol/L)和V-G溶液孵育30、60、120min后應用試劑盒測定NO的生成量,內皮細胞的總一氧化氮合酶(tNoS)活性,并用RT-PCR方法測定對內皮細胞一氧化氮合酶(eNOS)mRNA表達的影響.結果 17β-E2、辛伐他汀和V-G溶液作用30、60和120 min后可以使NO的生成量增加(P<0.05);17β-E2和辛伐他汀在作用30、60min時能夠增加tNOS活性,但作用時120mintNOS的活性降低(P<0.05);V-G溶液在作用30、60和120 min時均能夠增加HUVECs的tNOS活性(P<0.05);17β-E2、辛伐他汀和v-G溶液在作用120min后對eNOS的mRNA水平無影響(P>0.05).結論 短時間內17β-E2、辛伐他汀和v-G溶液能夠影響內皮細胞的tNos,增加內皮細胞的NO合成功能,V-G溶液的保護效果要優于前兩者. 英文摘要： Objective To investigate the effect of 17β-estrogen(E2), simvastatin and verapamil-nitrogtycerin (V-G) solution on the synthesise of NO in human umbilical vein cells (HUVECs). Methods HUVECs were treated with 17β-E2, simvastatin and V-G solution for 30 min, 60 min and 120 min, respectively. NO production and tNOS activity were measured with NO and tNOS re- agent box. levels of mRNA of endothelial nitric oxide synthase(eNOS) were examined with reverse transcriplion polymerase chain re- action (RT-PCR) after the HUVECs were incubated for 120 min. Results There was a significant increase of NO production after treating HUVECs with 17β-E2, simvastatin and V-G solution for 30, 60 and 120 minutes. The level of tNOS activity was significantly increased after treated with 17β-E2 and simvastatin for 30 and 60 minutes, but it declined after 120 minutes. V-G solution could in- crease tNOS activity at 3 time points. There were no difference in eNOS mRNA levels among 17β-E2, simvastatin and V-G solution at 120 min (P>0.05). Conclusion NO production of HUVECs could be increased by 17β-E2, simvastatin and V-G solution.
Tag: 臍帶血| 臍帶| 臍帶幹細胞| 脂肪幹細胞| 胎盤幹細胞| 羊胎水| 抗衰老|
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